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Research Updates

Hepatoprotective properties of oleanolic and ursolic acids

Objective: To estimate to what extent the mixture of ursolic acid and oleanolic acid, in addition to the antitubercular standard regime, affects the hepatotoxicity profile.
Methods: Liver injury was induced in male BALB/c mice by administering, per os and daily for 11 weeks, a combination of anti-Tubercular (anti-TB) agents Rifampicin (10 mg/ kg), Isoniazid (10 mg/kg), and Pyrazinamide (30 mg/kg). The ursolic acid and oleanolic acid mixture at doses of 100 or 200 mg/mouse/day was subcutaneously injected throughout the entire study period (11 weeks). Biochemical and hematological analysis was supplemented by liver histological examination.
Results: Animals treated with the mixture of triterpenic acids exhibited significantly decreased aspartate transaminase and alanine aminotransferase levels and amelioration of the histopathological alterations produced by the anti-TB drugs.
Conclusions: The triterpene mixture was able to prevent the steatosis induced by the anti-TB drugs.

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Fused core technology in HPLC columns

Turmeric has been used since ancient times as a seasoning, preservative and coloring agent as well as folk medicine. In turmeric rhizomes there are three main cucuminoids: Curcumindemethoxycurcumin and bisdemethoxycurcumin. Recent development of fused- core technology in HPLC columns is enabling faster and highly efficient separations.

The authors have used this technology and evaluated for the development of fast method for the analysis of main curcurminoids present in the extract. Step-by- step optimization strategy of chromatographic parameters was used to develop a fast and reproducible analysis method for the determination of curcuminoids. Separation of these three curcuminoids was achieved in approximately 1.3 min and the total analysis time, including column clean-up and re-equilibration times, was 7 min. The optimized method showed an excellent chromatographic performance in term of resolution, peak symmetry, repeatability, reproducibility, selectivity, and quantification and detection levels and was successfully used for the analysis of different real samples with similar performance. Based on the results gathered during the method development, it's clear that fused-core column technology has a great potential to deliver faster and more sensitive methods for the analysis of curcuminoids and other natural products.

This journal is published in Food chemistry

 

Development and validation of liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to quantify Bacopaside I in Mouse plasma and brain homogenate

Bacopaside I (BP-I) is the major pseudojujubogenin glycoside of Bacopa monnieri extract which has been widely used as a nerve tonic to improve the memory and intellect of human beings from ancient times. A selective and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of BP-I in mouse plasma and brain homogenate has been developed and validated. The method fulfilled US Food and Drug Administration (USFDA) guidelines for bioanalytical method validation in terms of selectivity, sensitivity, linearity, accuracy, precision, matrix effect, dilution integrity, carry-over effect and stability. Further, the method was successfully applied to execute the plasma pharmacokinetics and brain distribution of BP-I in Swiss-albino mice following intravenous administration.

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18α-Glycyrrhetinic acid (18α-GA), a bioactive component of Glycyrrhiza glabra, declines immunological responses mediated by dendritic cells

18α-Glycyrrhetinic acid (18α-GA) has been shown in vitro immunomodulatory effects on dendritic cells (DCs). 18α-GA was intraperitoneally administered to mice and splenic DCs were evaluated for expression of co-stimulatory molecules using flow cytometry. Isolated DCs were added to mixed lymphocyte reaction (MLR) and the proliferation of T cells was measured using BrdU assay. The level of IFN-γ in the MLR supernatant was determined by enzyme-linked immunosorbent assay. The in vivo effect of isolated DCs on antigen-specific delayed type hypersensitivity (DTH) response, and the number of regulatory T (Treg) cells in mice spleen by flow cytometry were investigated.

In DTH test, DCs isolated from 18α-GA-treated mice significantly suppressed antigen-specific cell mediated immune response (3.3 ± 1 mm in test group versus 6.5 ± 1.2 mm in control group, ρ < 0.01). The percentage of Treg cells in spleen of 18α-GA-treated mice (6.37% ± 2.3%) was lower than that of control group (13.85% ± 0.4%, ρ < 0.05). In vivo administration of 18α-GA resulted in inhibition of DCs maturation and T cell-mediated responses, the effects that may candidate this compound for its possible benefits in immune-mediated diseases.   

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