Phyto Compounds

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Development and validation of liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to quantify Bacopaside I in Mouse plasma and brain homogenate

Bacopaside I (BP-I) is the major pseudojujubogenin glycoside of Bacopa monnieri extract which has been widely used as a nerve tonic to improve the memory and intellect of human beings from ancient times. A selective and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of BP-I in mouse plasma and brain homogenate has been developed and validated. The method fulfilled US Food and Drug Administration (USFDA) guidelines for bioanalytical method validation in terms of selectivity, sensitivity, linearity, accuracy, precision, matrix effect, dilution integrity, carry-over effect and stability. Further, the method was successfully applied to execute the plasma pharmacokinetics and brain distribution of BP-I in Swiss-albino mice following intravenous administration.


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